[1] Liu Shuangyue Xu Xiaoying Zhang Yulian Ai Shuangchun,.Effect of Electroacupuncture on Expression of GDNF in Ischemic Penumbra of MCAO Model Rats [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2019, (05): 58-63. [doi: 10.13424/j.cnki.jsctcm.2019.05.014]
Effect of Electroacupuncture on Expression of GDNF in Ischemic Penumbra of MCAO Model Rats
- Article number:
- 2096-1340(2019)05-0058-06
- Keywords:
- electro - acupuncture; stroke; ischemia reperfusion injury; GDNFmRNA; NF-κB
- ZTFLH:
- R245.31+9
- Code:
- A
- Abstract:
- To observe the effect of electro - acupuncture at Shuigou, Neiguan, Sanyinjiao and Weizhong points on the expression of glial cell line derived neurotrophic factor ( GDNF) in ischemic penumbra of rats with cerebral ischemia - reperfusion, and to explore its mechanism of reducing cerebral ischemia - reperfusion injury. Methods 120 adult male Sprague - Dawley ( SD) rats of clean grade were randomly divided into five groups: blank group, sham operation group, model group, electro - acupuncture group and inhibitor group, with 24 rats in each group. The middle cerebral artery occlusion ( MCAO) model was established by using Longa’ s modified thread embolization method. In the electro - acupuncture group, rats were stimulated by electro - acupuncture at Shuigou, Neiguan, Sanyinjiao and Weizhong points once a day for 28 days. The inhibitor group was intraperitoneally injected with NF - κB inhibitor PDTC ( 120 mg/kg) , and then electro - acupuncture was stimulated once a day for 28 days. Real - time fluorescence quantitative polymerase chain reaction ( PCR) was used to detect the mRNA expression of GDNF in the ischemic penumbra of rat brain.Results Compared with the model group, the expression of GDNF mRNA in the electroacupuncture group decreased on the 3rd day after treatment, and the expression increased on the 7th, 14th and 28th days, the difference was statistically significant ( P <0. 05) . The expression of GDNF in NF - kappa B inhibitor group decreased on the 3rd and 7th day ( P < 0.05) , but there was no significant difference on the 14th and 28th day ( P > 0. 05) . The expression of GDNF mRNA was significantly down - regulated in the inhibitor group at the four observation time points after treatment, and the difference was statistically significant ( P <0. 05) . Conclusion Electroacupuncture can up - regulate the expression of GDNFmRNA and protect the rats from ischemia - reperfusion. The mechanism may be related to the activity of NF - kB signaling pathway.
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Memo
基金项目：绵阳市科技局资助项目（２０１５ｙｙ０３７）