[1] Zhang Li Zhou Jin Zhao Yue,.Study on the Mechanism of Zhuyu Sanjie Granule in the Treatment of Uterine Fibroid in Rats [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2019, (04): 35-38. [doi: 10.13424/j.cnki.jsctcm.2019.04.009]
Study on the Mechanism of Zhuyu Sanjie Granule in the Treatment of Uterine Fibroid in Rats
- Article number:
- 2096 -1340( 2019) 04 -0035 -04
- Keywords:
- Zhuyu Sanjie Granule; uterine fibroid; estradiol ( E2) and progesterone ( P) ; immune level
- ZTFLH:
- R211.76
- Code:
- A
- Abstract:
- Objective To observe the effect of Zhuyu Sanjie Granule on the levels of sex hormones and cytokines in uterine fibroid rats, and to explore its mechanism of action. Methods SD rats were randomly divided into blank group,model group, Guizhi Fuling group, Zhuyu Sanjie Granule high and low dose group ( 360, 180mg? kg -1) . Rat model of uterine fibroids was induced by intramuscular injection of estradiol benzoate and progesterone. The levels of estradiol ( E2) and progesterone ( P) were measured by radioimmunoassay. Serum IL -2 and tumor necrosis factor content( TNF -α) were detected by ELISA , and uterine histopathological examination was performed. Results The rats in the model group showed an increase in uterine organ coefficient and a decrease in ovarian organ coefficient; E2 and P levels increased, serum IL -2 levels decreased, and TNF - α levels increased; uterine smooth muscle layer thickened and arranged disorderly The nucleus is enlarged. Compared with the model group, the uterine coefficient decreased, the ovarian coefficient increased, the serum E2 and P decreased, the serum IL - 2 level increased and the TNF - alpha level decreased in the high and low dose groups of Zhuyu Sanjie Granule. Conclusion Zhuyu Sanjie Granule can improve the pathological morphology of uterine fibroids, reduce serum E2 and P, and regulate immune function. These may be the mechanism of its treatment of uterine fibroids.
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Memo
基金项目：陕西省中医药管理局科研项目（ＺＹＭＳ０１５）