[1] Nie Yusong Yan Miao Xie Hui Fan Xinrong,.Preparing Method of Obese Rat Model with Spleen Deficiency and Phlegm Dampness [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2019, (03): 68-72,111. [doi: 10.13424/j.cnki.jsctcm.2019.03.019]
Preparing Method of Obese Rat Model with Spleen Deficiency and Phlegm Dampness
- Article number:
- 2096-1340(2019)03-0068-06
- Keywords:
- spleen deficiency and phlegm dampness; obesity; rat model
- ZTFLH:
- R259
- Code:
- A
- Abstract:
- Objective To explore preparing method of obese rat model with spleen deficiency and phlegm dampness. Methods Twenty - four SD male rats were randomly divided into normal group ( n =8) , model A group ( n =8) and model B group ( n =8) . Models A and B were treated with different intervention sequences. The general behavioral changes and hair color, stool, body mass, mental state, and spleen deficiency scores were observed. Total cholesterol ( TC) , triglyceride ( TG) , high density lipoprotein cholesterol ( HDL - C) , low density lipoprotein cholesterol ( LDL - C) , glutathione transaminase ( ALT) were measured and liver pathology was observed. Results After feeding with high - fat diet,the weight of model group was higher than that of normal group ( P < 0. 05) ; after intervention, the model group rats were obese with greasy coat, sluggish activity and loose stool, easy to get tired and lying down, which accorded with the general manifestation of spleen deficiency and phlegm dampness; the model group A and B all had different degrees of fatty liver, and the liver function damage of model group A rats was more obvious. Conclusion The obese rats with spleen deficiency and phlegm dampness can be prepared by feeding with high fat diet, excessive fatigue and cold attack, but there are still some places to be improved; and the method of model group A is more satisfied with the model requirementsthan that of model group B, and meets the expected results.
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