[1] Shen Xiaohong Sun Yuanyuan Cheng Guang,.Effect of Chinese Prescriptions of Removing Toxicity and Dredging Collateral as well as Nourishing Yin on Human Gastric Cancer Cell BGC -823 [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2019, (01): 86-90. [doi: 10.13424/j.cnki.jsctcm.2019.01.027]
Effect of Chinese Prescriptions of Removing Toxicity and Dredging Collateral as well as Nourishing Yin on Human Gastric Cancer Cell BGC -823
- Article number:
- 2096-1340(2019)01-0086-05
- Keywords:
- BGC -823; ERK; MMP9; removing toxicity and dredging collateral; boasting qi and nourishing yin
- ZTFLH:
- R285.5
- Code:
- A
- Abstract:
- To investigate the effect of Chinese prescriptions of removing toxicity and dredging collateral as well as nourishing yin on human gastric cancer cell BGC -823. Method: Serum was obtained by intragastric administration in mice and sterilized to intervene in gastric cancer cell BGC - 823. Cell proliferation, apoptosis and invasiveness were detected by cell proliferation test and Western blot test. Result: Cell proliferation test showed that there was no significant change in cell proliferation in normal saline group compared with blank control group ( P >0. 05) . Cell proliferation in removing toxicity and dredging collateral group and nourishing yin group were significantly lower than that in normal saline group ( P < 0. 05) . Western blot results showed that the expressions of ERK and MMP9 protein in removing toxicity and dredging collateral group and nourishing yin group were significantly lower than that in normal saline group ( P < 0.05) . Bax protein expression showed a significant increase ( P <0. 05) . Compared with saline group, E - Cad protein expression increased significantly in nourishing yin group( P < 0. 05) . Conclusion: Chinese prescriptions of removing toxicity and dredging collateral as well as nourishing yin can affect the expression levels of ERK, MMP9, BAX and E – Cad proteins, inhibit the proliferation of gastric cancer cells BGC823, promote their apoptosis, and play an anti - cancer effect to a certain extent.
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