[1] Xu Bing, Zhang Lijun, Guo Xiaolan, et al. Experimental Study on the Inhibitory Effect of Grifola Frondosa on H22 Liver Cancer Xenografts [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2018, (06): 97-100. [doi: 10.13424/j.cnki.jsctcm.2018.06.031]
Experimental Study on the Inhibitory Effect of Grifola Frondosa on H22 Liver Cancer Xenografts
- Article number:
- 2096－1340(2018)06－0097－04
- ZTFLH:
- Ｒ735. 7
- Code:
- A
- Abstract:
- Objective: To investigate the inhibitory effects of different doses of grifola frondosa on H22 hepatoma xenografts． Method: The H22 tumor － bearing mouse model was established． Grifola frondosa were divided into low and high doses with 300，600 mg/( kg． d) for each． The mice were treated with different doses of grifola frondosa from the next day of modeling． The mice in the control group were given the same amount of normal saline for 3 weeks． The size of tumor lump was measured regularly． 12 hours after the last administration，anesthetized mice，blood samples were taken， and the level of lymphocyte subsets in peripheral blood of mice was detected by flow cytometry; spleen was taken to detect the proliferative ability of splenic lymphocytes and NK cell killing activity; thymus and spleen were weighed to calculate the spleen and thymus index; tumor mass was stripped，tumor was weighed，and tumor inhibition rate was calculated． Ｒesult: Different doses of grifola frondosa could significantly inhibit the growth of tumor in mice，especially in high doses， the inhibition rates of low doses and high doses were 39． 28% and 55． 31%， respectively; grifola frondosa could also enhance the proliferation of splenic lymphocytes in tumor － bearing mice; the killing activity of NK cells was significantly improved，compared with the model group， the difference was significant( P ＜0． 05) ． Grifola frondosa could promote the proliferation of T cells，especially CD8 + T cells，and correct the ratio of CD4 / CD8 in tumor － bearing mice，especially in large doses，which was significantly different from the model group ( P ＜ 0． 05) ． Conclusion: Grifola frondosa can improve the immune function of tumor bearing mice and has anti － tumor effect，which could be achieved through grifola frondosa’ s function in maintaining the stability of the organ index，protecting the immune organs of mice，controlling the growth of tumor，enhancing the proliferation of lymphocytes，enhancing the killing activity of NK cells，and correcting the disorder of CD4 / CD8 ratio in tumor － bearing mice．
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[1２]彭植强，苏林，陈时新．自拟益气健脾固本方联合胸腺 五肽穴位注射治疗癌症相关性疲劳的临床研究［Ｊ]．现 代中医药，２０１８，３８（５）：４４－４７． （收稿日期：２０１８－０４－１
Memo
基金项目:陕西省自然科学基础研究项目( 2016JM8150); 陕西省教育厅自然科学基础研究项目( 11JK0686) 陕西中 医药大学科研基金( 2016PY08)收稿日期:2018 －04 －10 编辑:文颖娟