[1] Wang Jing, Zhao Chongbo, Bo Xihui, et al. Study on the Quality Standard of Tianren Hypnotic Granules [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2018, (02): 97-101. [doi: 10.13424/j.cnki.jsctcm.2018.02.029]
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Journal of Shaanxi University of Traditional Chinese Medicine[ISSN: 2096-1340/ CN: 61-1501/R]
Volumes & Issues:
2018 02
pagination:
97-101
Column:
Publication date:
2018-03-10
- Article number:
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2096-1340(2018)02-0097-05
- Author(s):
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Wang Jing; Zhao Chongbo; Bo Xihui; Zhao Xiaoxiao; Zhang Siqian; Wang Changli
-
Shaanxi University of Chinese Medicine,Xianyang 712046,China)
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- Keywords:
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Tianren hypnotic granules; gastrodin; TLC; HPLC
- ZTFLH:
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R284. 1
- DOI:
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10.13424/j.cnki.jsctcm.2018.02.029
- Code:
-
A
- Abstract:
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To establish quality standard for Tianren hypnotic granules. Method: gastrodia elata and
spine date seed in Tianren hypnotic granules were identified by TLC. The content of gastrodin in the product was deter-
mined by high performance liquid chromatography. The mobile phase conditions were HYPERSIL C18 column,mobile
phase acetonitrile - 0. 05% phosphoric acid solution ( 3: 97) ,flow rate of 0. 5 ml / min,column temperature of 25 ℃ ,
detection wavelength of 220 nm. Result: TLC spots were clear and well separated. The mass concentration of gastrodin
showed a good linear relationship with the peak area in the range of 0. 05 ~ 0. 30 mg / m L ( r = 0. 9999) ,and the average
recovery rate of gastrodin was 100. 67% ,RSD = 1. 39% ( n = 6) . Conclusion: The qualitative and quantitative methods
established in this study are simple and feasible,and can be used for the quality control of Tianren hypnotic granules.