[1] Shi Xiaofeng Huang Jingjing Shi Ya Ding Zhishan Cheng Dongqing,.On AMF Diversity of Pinellia Ternate [J]. Journal of Shaanxi University of Traditional Chinese Medicine, 2017, (03): 75-81. [doi: 10.13424/j.cnki.jsctcm.2017.03.026]
On AMF Diversity of Pinellia Ternate
- Article number:
- 2096－1340(2017)03－0075－07
- Keywords:
- pinellia ternate; AMF; spores; infection rate; diversity
- ZTFLH:
- Ｒ931.2
- Code:
- A
- Abstract:
- To study the diversity of AMF ( Arbuscular mycorrhizal fungi) in pinellia ternate． Method: Pinellia ternate and rhizosphere soil were collected from Hangzhou in Zhenjiang Province ( including Hupao， Xiamanjuelong and Fuyang) and Guiyang in Guizhou Province． The tubers were cut into slices and roots made into 1cm long segments， which were colored and decolorized by lactated glycerid acid fuchsin to observe their AMF’ s morphological structure in plantlets of pinellia ternate． The AMF spores in rhizosphere soils were isolated by the method of wet screening and sucrose centrifugation and identified to analyze the diversity of the spores from different regions． Ｒesults: There were large quantities of hypha and vesicle between and inside cells of cortical tissues as well as the typical branched structure in pinellia ternate． A lot of AMF spores in rhizosphere soils were being existent． The identification showed that there were 20 kinds of pinellia ternate belonging to 3 genera， among which 16 kinds attributes to genus Glomus， 3 to genus Acaulospora and 1 to genus Scutellospora， taking up 80． 0% ， 15． 0% and 5． 0% respectively． Discussion: AMF can be formed in all regions of pinellia ternate and AMF spores in rhizosphere soils are highly diverse， in which the genus Glomus are predominant bacterium．
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Memo
收稿日期:2016－06－27编辑:文颖娟
基金项目:中医药行业科研专项子课题(201507002－1－11)
通讯作者:程东庆(1971－)，副教授，硕士研究生导师，主要研究方向:药学、微生物学和免疫学研究。E－mail:
B2006069@163．com